A Requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3' end formation.
RNA synthesis and processing are coordinated by proteins that associate with RNA polymerase II (pol II) during transcription elongation. The yeast Paf1 complex interacts with RNA pol II and mediates histone modifications during elongation. To elucidate the functions of this complex, we isolated missense mutations in the gene encoding the ... Rtf1 subunit and used them to identify functionally interacting proteins. We identified NAB3 as a dosage suppressor of rtf1. Nab3, together with Nrd1, directs 3' end formation of nonpolyadenylated RNA pol II transcripts, such as snoRNAs. Deletion of Paf1, but not the Set1, Set2, or Dot1 histone methyltransferases, causes accumulation of snoRNA transcripts that are extended at their 3' ends. The Paf1 complex associates with and facilitates Nrd1 recruitment to the SNR47 gene, suggesting a direct involvement in 3' end formation. Our results reveal a posttranscriptional function for the Paf1 complex, which appears unrelated to its role in histone methylation.
Mesh Terms:
Gene Expression Regulation, Mutation, Nuclear Proteins, RNA 3' End Processing, RNA Polymerase II, RNA, Messenger, RNA, Small Nucleolar, RNA-Binding Proteins, Ribonucleoproteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, TATA-Box Binding Protein, Transcription Factors, Transcription, Genetic
Gene Expression Regulation, Mutation, Nuclear Proteins, RNA 3' End Processing, RNA Polymerase II, RNA, Messenger, RNA, Small Nucleolar, RNA-Binding Proteins, Ribonucleoproteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, TATA-Box Binding Protein, Transcription Factors, Transcription, Genetic
Mol. Cell
Date: Oct. 28, 2005
PubMed ID: 16246725
View in: Pubmed Google Scholar
Download Curated Data For This Publication
68153
Switch View:
- Interactions 4