A functional network involved in the recycling of nucleocytoplasmic pre-60S factors.

Eukaryotic pre-ribosomes go through cytoplasmic maturation steps before entering translation. The nucleocytoplasmic proteins participating in these late stages of maturation are reimported to the nucleus. In this study, we describe a functional network focused on Rei1/Ybr267w, a strictly cytoplasmic pre-60S factor indirectly involved in nuclear 27S pre-ribosomal RNA processing. In ...
the absence of Rei1, the nuclear import of at least three other pre-60S factors is impaired. The accumulation in the cytoplasm of a small complex formed by the association of Arx1 with a novel factor, Alb1/Yjl122w, inhibits the release of the putative antiassociation factor Tif6 from the premature large ribosomal subunits and its recycling to the nucleus. We propose a model in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation.
Mesh Terms:
Active Transport, Cell Nucleus, Carrier Proteins, Cell Nucleus, Intermediate Filament Proteins, Membrane Transport Proteins, Models, Biological, Mutation, Phosphoproteins, Polyribosomes, Protein Binding, RNA-Binding Proteins, Receptors, Cytoplasmic and Nuclear, Ribosomal Proteins, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Two-Hybrid System Techniques
J. Cell Biol.
Date: May. 08, 2006
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