Saccharomyces cerevisiae GTPase complex: Gtr1p-Gtr2p regulates cell-proliferation through Saccharomyces cerevisiae Ran-binding protein, Yrb2p.
A Gtr1p GTPase, the GDP mutant of which suppresses both temperature-sensitive mutants of Saccharomyces cerevisiae RanGEF/Prp20p and RanGAP/Rna1p, was presently found to interact with Yrb2p, the S. cerevisiae homologue of mammalian Ran-binding protein 3. Gtr1p bound the Ran-binding domain of Yrb2p. In contrast, Gtr2p, a partner of Gtr1p, did not ... bind Yrb2p, although it bound Gtr1p. A triple mutant: yrb2delta gtr1delta gtr2delta was lethal, while a double mutant: gtr1delta gtr2delta survived well, indicating that Yrb2p protected cells from the killing effect of gtr1delta gtr2delta. Recombinant Gtr1p and Gtr2p were purified as a complex from Escherichia coli. The resulting Gtr1p-Gtr2p complex was comprised of an equal amount of Gtr1p and Gtr2p, which inhibited the Rna1p/Yrb2 dependent RanGAP activity. Thus, the Gtr1p-Gtr2p cycle was suggested to regulate the Ran cycle through Yrb2p.
Mesh Terms:
Cell Proliferation, GTP Phosphohydrolases, GTP-Binding Proteins, GTPase-Activating Proteins, Macromolecular Substances, Monomeric GTP-Binding Proteins, Nuclear Proteins, Protein Interaction Mapping, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, ran GTP-Binding Protein
Cell Proliferation, GTP Phosphohydrolases, GTP-Binding Proteins, GTPase-Activating Proteins, Macromolecular Substances, Monomeric GTP-Binding Proteins, Nuclear Proteins, Protein Interaction Mapping, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, ran GTP-Binding Protein
Biochem. Biophys. Res. Commun.
Date: Oct. 21, 2005
PubMed ID: 16143306
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