Rad4-Rad23 interaction with SWI/SNF links ATP-dependent chromatin remodeling with nucleotide excision repair.
Chromatin rearrangement occurs during nucleotide excision repair (NER). Here we show that Snf6 and Snf5, two subunits of the SWI/SNF chromatin-remodeling complex in Saccharomyces cerevisiae, copurify with the NER damage-recognition heterodimer Rad4-Rad23. This interaction between SWI/SNF and Rad4-Rad23 is stimulated by UV irradiation. We demonstrate that NER in the transcriptionally ... silent, nucleosome-loaded HML locus is reduced in yeast cells lacking functional SWI/SNF. In addition, using a restriction enzyme accessibility assay, we observed UV-induced nucleosome rearrangement at the silent HML locus. Notably, this rearrangement is markedly attenuated when SWI/SNF is inactivated. These results indicate that the SWI/SNF chromatin-remodeling complex is recruited to DNA lesions by damage-recognition proteins to increase DNA accessibility for NER in chromatin.
Mesh Terms:
Adenosine Triphosphate, Cells, Cultured, Chromatin, Chromatin Assembly and Disassembly, Chromosomal Proteins, Non-Histone, DNA Repair, DNA-Binding Proteins, Models, Biological, Models, Molecular, Saccharomyces cerevisiae Proteins, Transcription Factors
Adenosine Triphosphate, Cells, Cultured, Chromatin, Chromatin Assembly and Disassembly, Chromosomal Proteins, Non-Histone, DNA Repair, DNA-Binding Proteins, Models, Biological, Models, Molecular, Saccharomyces cerevisiae Proteins, Transcription Factors
Nat. Struct. Mol. Biol.
Date: Oct. 01, 2006
PubMed ID: 17013386
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