The Arabidopsis SERK1 protein interacts with the AAA-ATPase AtCDC48, the 14-3-3 protein GF14lambda and the PP2C phosphatase KAPP.
Leucine-rich repeat (LRR)-containing transmembrane receptor-like kinases (RLKs) are important components of plant signal transduction. The Arabidopsis thaliana somatic embryogenesis receptor-like kinase 1 (AtSERK1) is an LRR-RLK proposed to participate in a signal transduction cascade involved in embryo development. By yeast two-hybrid screening we identified AtCDC48, a homologue of the mammalian ... AAA-ATPase p97 and GF14lambda, a member of the Arabidopsis family of 14-3-3 proteins as AtSERK1 interactors. In vitro, the AtSERK1 kinase domain is able to transphosphorylate and bind both AtCDC48 and GF14lambda. In yeast, AtCDC48 interacts with GF14lambda and with the PP2C phosphatase KAPP. In plant protoplasts AtSERK1 interacts with GF14lambda.
Mesh Terms:
Adenosine Triphosphatases, Arabidopsis, Arabidopsis Proteins, Binding Sites, Cell Cycle Proteins, Gene Expression Profiling, Phosphoprotein Phosphatases, Phosphorylation, Protein Binding, Protein Kinases, Protoplasts, Recombinant Fusion Proteins, Signal Transduction, Trans-Activators, Two-Hybrid System Techniques
Adenosine Triphosphatases, Arabidopsis, Arabidopsis Proteins, Binding Sites, Cell Cycle Proteins, Gene Expression Profiling, Phosphoprotein Phosphatases, Phosphorylation, Protein Binding, Protein Kinases, Protoplasts, Recombinant Fusion Proteins, Signal Transduction, Trans-Activators, Two-Hybrid System Techniques
Planta
Date: Jun. 01, 2005
PubMed ID: 15592873
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