Rad52 and Rad59 exhibit both overlapping and distinct functions.
Homologous recombination is an important pathway for the repair of DNA double-strand breaks (DSBs). In the yeast Saccharomyces cerevisiae, Rad52 is a central recombination protein, whereas its paralogue, Rad59, plays a more subtle role in homologous recombination. Both proteins can mediate annealing of complementary single-stranded DNA in vitro, but only ... Rad52 interacts with replication protein A and the Rad51 recombinase. We have studied the functional overlap between Rad52 and Rad59 in living cells using chimeras of the two proteins and site-directed mutagenesis. We find that Rad52 and Rad59 have both overlapping as well as separate functions in DSB repair. Importantly, the N-terminus of Rad52 possesses functions not supplied by Rad59, which may account for its central role in homologous recombination.
Mesh Terms:
Amino Acid Sequence, DNA Breaks, Double-Stranded, DNA Repair, DNA Replication, DNA, Fungal, DNA, Single-Stranded, DNA-Binding Proteins, Genetic Complementation Test, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, Protein Conformation, Rad51 Recombinase, Rad52 DNA Repair and Recombination Protein, Recombination, Genetic, Replication Protein A, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid
Amino Acid Sequence, DNA Breaks, Double-Stranded, DNA Repair, DNA Replication, DNA, Fungal, DNA, Single-Stranded, DNA-Binding Proteins, Genetic Complementation Test, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutation, Protein Conformation, Rad51 Recombinase, Rad52 DNA Repair and Recombination Protein, Recombination, Genetic, Replication Protein A, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid
DNA Repair (Amst.)
Date: Jan. 04, 2007
PubMed ID: 16987715
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