The scaffolding protein CASK mediates the interaction between rabphilin3a and beta-neurexins.
CASK, a member of the membrane-associated guanylate kinase (MAGUK) superfamily, binds to the carboxyl-terminus of beta-neurexins on the intracellular side of the presynaptic membrane. The guanylate kinase-like (GUK) domains of MAGUKs lack kinase activities, but might be important for mediating specific protein-protein interaction. By a yeast two-hybrid approach, we identified ... an interaction between the GUK domain of CASK and the C2B domain of rabphilin3a, a presynaptic protein involved in synaptic vesicle exocytosis. The interaction was confirmed by in vitro GST pull-down and co-immunoprecipitation assays. It was proposed that presynaptic vesicles might be guided to the vicinity of points of exocytosis defined by beta-neurexins via the interaction between rabphilin3a-CASK-beta-neurexins.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Animals, Blotting, Western, COS Cells, Calcium-Calmodulin-Dependent Protein Kinases, Exocytosis, Glutathione Transferase, Guanylate Kinase, Nerve Tissue Proteins, Nucleoside-Phosphate Kinase, Precipitin Tests, Protein Binding, Protein Isoforms, Protein Structure, Tertiary, Recombinant Fusion Proteins, Synaptic Vesicles, Two-Hybrid System Techniques, Vesicular Transport Proteins, rab GTP-Binding Proteins
Adaptor Proteins, Signal Transducing, Animals, Blotting, Western, COS Cells, Calcium-Calmodulin-Dependent Protein Kinases, Exocytosis, Glutathione Transferase, Guanylate Kinase, Nerve Tissue Proteins, Nucleoside-Phosphate Kinase, Precipitin Tests, Protein Binding, Protein Isoforms, Protein Structure, Tertiary, Recombinant Fusion Proteins, Synaptic Vesicles, Two-Hybrid System Techniques, Vesicular Transport Proteins, rab GTP-Binding Proteins
FEBS Lett.
Date: May. 25, 2001
PubMed ID: 11377421
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