Golgin-84 is a rab1 binding partner involved in Golgi structure.
Members of the golgin family of coiled-coil proteins have been implicated in the tethering of vesicles to Golgi membranes and cisternal membranes to each other. Many also bind to rab GTPases. Golgin-84 is a membrane-anchored golgin that we now show binds preferentially to the GTP form of the rab1 GTPase. ... It is also present throughout the Golgi stack by immuno-EM. Antibodies to golgin-84 inhibit stacking of cisternal membranes in a cell-free assay for Golgi reassembly, whereas the cytoplasmic domain of golgin-84 stimulates stacking and increases the length of re-assembled stacks. Transient expression of golgin-84 in NRK cells helps prevent the disassembly of the Golgi apparatus normally triggered by treatment with brefeldin A. Together these data suggest that golgin-84 is involved in generating and maintaining the architecture of the Golgi apparatus.
Mesh Terms:
Animals, Cells, Cultured, Cryoelectron Microscopy, Golgi Apparatus, Liver, Membrane Proteins, Microscopy, Immunoelectron, Mitosis, Molecular Sequence Data, Protein Binding, Rats, rab1 GTP-Binding Proteins
Animals, Cells, Cultured, Cryoelectron Microscopy, Golgi Apparatus, Liver, Membrane Proteins, Microscopy, Immunoelectron, Mitosis, Molecular Sequence Data, Protein Binding, Rats, rab1 GTP-Binding Proteins
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Date: Mar. 01, 2003
PubMed ID: 12656988
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