The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation.

Fragmentation of the mammalian Golgi apparatus during mitosis requires the phosphorylation of a specific subset of Golgi-associated proteins. We have used a biochemical approach to characterize these proteins and report here the identification of golgin-84 as a novel mitotic target. Using cryoelectron microscopy we could localize golgin-84 to the cis-Golgi ...
network and found that it is enriched on tubules emanating from the lateral edges of, and often connecting, Golgi stacks. Golgin-84 binds to active rab1 but not cis-Golgi matrix proteins. Overexpression or depletion of golgin-84 results in fragmentation of the Golgi ribbon. Strikingly, the Golgi ribbon is converted into mini-stacks constituting only approximately 25% of the volume of a normal Golgi apparatus upon golgin-84 depletion. These mini-stacks are able to carry out protein transport, though with reduced efficiency compared with a normal Golgi apparatus. Our results suggest that golgin-84 plays a key role in the assembly and maintenance of the Golgi ribbon in mammalian cells.
Mesh Terms:
Animals, Autoantigens, Endoplasmic Reticulum, Eukaryotic Cells, Gene Expression Regulation, Golgi Apparatus, Hela Cells, Humans, Intracellular Membranes, Membrane Proteins, Microscopy, Electron, Microtubule Proteins, Mitosis, Phosphoproteins, Protein Transport, RNA, Small Interfering, Rats, Recombinant Fusion Proteins, Subcellular Fractions, Viral Proteins, rab1 GTP-Binding Proteins
J. Cell Biol.
Date: Jan. 20, 2003
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