Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function.
The anaphase-promoting complex/cyclosome (APC/C) is a conserved multisubunit ubiquitin ligase required for the degradation of key cell cycle regulators. The APC/C becomes active at the metaphase/anaphase transition and remains active during G(1) phase. One mechanism linked to activation of the APC/C is phosphorylation. Although many sites of mitotic phosphorylation have ... been identified in core components of the APC/C, the consequence of any individual phosphorylation event has not been elucidated in vivo. In this study, we show that Hcn1 is an essential core component of the fission yeast APC/C and is critical for maintaining complex integrity. Moreover, Hcn1 is a phosphoprotein in vivo. Phosphorylation of Hcn1 occurs at a single Cdk1 site in vitro and in vivo. Mutation of this site to alanine, but not aspartic acid, compromises APC/C function and leads to a specific defect in the completion of cell division.
Mesh Terms:
Alanine, Amino Acid Substitution, Cell Cycle Proteins, Fungal Proteins, G2 Phase, Gene Deletion, Green Fluorescent Proteins, Phosphorylation, Repressor Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Ubiquitin-Protein Ligase Complexes
Alanine, Amino Acid Substitution, Cell Cycle Proteins, Fungal Proteins, G2 Phase, Gene Deletion, Green Fluorescent Proteins, Phosphorylation, Repressor Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Ubiquitin-Protein Ligase Complexes
J. Biol. Chem.
Date: Oct. 27, 2006
PubMed ID: 16950791
View in: Pubmed Google Scholar
Download Curated Data For This Publication
69859
Switch View:
- Interactions 23