Ras-activated endocytosis is mediated by the Rab5 guanine nucleotide exchange activity of RIN1.
RIN1 was originally identified by its ability to inhibit activated Ras and likely participates in multiple signaling pathways because it binds c-ABL and 14-3-3 proteins, in addition to Ras. RIN1 also contains a region homologous to the catalytic domain of Vps9p-like Rab guanine nucleotide exchange factors (GEFs). Here, we show ... that this region is necessary and sufficient for RIN1 interaction with the GDP-bound Rabs, Vps21p, and Rab5A. RIN1 is also shown to stimulate Rab5 guanine nucleotide exchange, Rab5A-dependent endosome fusion, and EGF receptor-mediated endocytosis. The stimulatory effect of RIN1 on all three of these processes is potentiated by activated Ras. We conclude that Ras-activated endocytosis is facilitated, in part, by the ability of Ras to directly regulate the Rab5 nucleotide exchange activity of RIN1.
Mesh Terms:
Animals, CHO Cells, Carrier Proteins, Catalytic Domain, Cricetinae, Endocytosis, Endosomes, Fibroblasts, Fungal Proteins, Gene Expression, Guanine Nucleotide Exchange Factors, Mice, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, rab GTP-Binding Proteins, rab5 GTP-Binding Proteins, ras Proteins
Animals, CHO Cells, Carrier Proteins, Catalytic Domain, Cricetinae, Endocytosis, Endosomes, Fibroblasts, Fungal Proteins, Gene Expression, Guanine Nucleotide Exchange Factors, Mice, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, rab GTP-Binding Proteins, rab5 GTP-Binding Proteins, ras Proteins
Dev. Cell
Date: Jul. 01, 2001
PubMed ID: 11703925
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