Definition of a large region of RAG1 that is important for coimmunoprecipitation of RAG2.
Interaction between the RAG1 and RAG2 proteins is probably critical for V(D)J recombination. Using a coimmunoprecipitation assay, we define a large region of RAG1 (amino acids 504-1008) that is sufficient for interaction with RAG2. This region comprises the C-terminal half of the RAG1 protein, and is within the region defined ... as the recombinationally active core. Deletion of either of two regions of RAG1 (amino acids 504-570 or 850-1008) causes a loss of interaction with RAG2. Loss of coimmunoprecipitation is also seen with RAG1 core proteins containing deletions of smaller stretches of amino acids (amino acids 506-511 or 545-550), emphasizing the importance of this region of RAG1 in forming a complex with RAG2. A variety of other small deletion mutations within the amino acid region 504-1008 also decrease coimmunoprecipitation of RAG2 with RAG1, indicating that much or all of this region is important for complex formation.
Mesh Terms:
Animals, Cell Nucleus, DNA-Binding Proteins, Homeodomain Proteins, Mice, Mutagenesis, Precipitin Tests, Proteins, Receptors, Antigen, T-Cell, Recombination, Genetic, Sequence Deletion, Transfection
Animals, Cell Nucleus, DNA-Binding Proteins, Homeodomain Proteins, Mice, Mutagenesis, Precipitin Tests, Proteins, Receptors, Antigen, T-Cell, Recombination, Genetic, Sequence Deletion, Transfection
J. Immunol.
Date: Mar. 01, 1997
PubMed ID: 9036966
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