Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system.
The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the "bait" in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated ... UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52's self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.
Mesh Terms:
Carrier Proteins, Chromosome Mapping, Chromosomes, Human, Pair 16, DNA Repair, DNA-Binding Proteins, Fungal Proteins, Humans, Ligases, Male, Molecular Sequence Data, Protein Binding, RNA, Messenger, Rad51 Recombinase, Rad52 DNA Repair and Recombination Protein, SUMO-1 Protein, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces pombe Proteins, Testis, Tumor Suppressor Protein p53, Ubiquitin-Conjugating Enzymes
Carrier Proteins, Chromosome Mapping, Chromosomes, Human, Pair 16, DNA Repair, DNA-Binding Proteins, Fungal Proteins, Humans, Ligases, Male, Molecular Sequence Data, Protein Binding, RNA, Messenger, Rad51 Recombinase, Rad52 DNA Repair and Recombination Protein, SUMO-1 Protein, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Schizosaccharomyces pombe Proteins, Testis, Tumor Suppressor Protein p53, Ubiquitin-Conjugating Enzymes
Genomics
Date: Oct. 15, 1996
PubMed ID: 8921390
View in: Pubmed Google Scholar
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