A novel nucleic acid-binding protein that interacts with human rad51 recombinase.
Using the yeast two-hybrid system, we isolated a cDNA encoding a novel human protein, named Pir51, that strongly interacts with human Rad51 recombinase. Analysis in vitro confirmed the interaction between Rad51 and Pir51. Pir51 mRNA is expressed in a number of human organs, most notably in testis, thymus, colon and ... small intestine. The Pir51 gene locus was mapped to chromosome 12p13.1-13. 2 by fluorescence in situ hybridization. The Pir51 protein was expressed in Escherichia coli and purified to near homogeneity. Biochemical analysis shows that the Pir51 protein binds both single- and double-stranded DNA, and is capable of aggregating DNA. The protein also binds RNA. The Pir51 protein may represent a new member of the multiprotein complexes postulated to carry out homologous recombination and DNA repair in mammalian cells.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Chromosomes, Human, Pair 12, DNA, DNA, Complementary, DNA-Binding Proteins, Genes, Hela Cells, Humans, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Organ Specificity, Protein Binding, RNA, RNA, Messenger, Rad51 Recombinase, Recombination, Genetic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Amino Acid Sequence, Base Sequence, Chromosomes, Human, Pair 12, DNA, DNA, Complementary, DNA-Binding Proteins, Genes, Hela Cells, Humans, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Organ Specificity, Protein Binding, RNA, RNA, Messenger, Rad51 Recombinase, Recombination, Genetic, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Nucleic Acids Res.
Date: Dec. 15, 1997
PubMed ID: 9396801
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