Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170.
Linkage of microtubules to special cortical regions is essential for cell polarization. CLIP-170 binds to the growing ends of microtubules and plays pivotal roles in orientation. We have found that IQGAP1, an effector of Rac1 and Cdc42, interacts with CLIP-170. In Vero fibroblasts, IQGAP1 localizes at the polarized leading edge. ... Expression of carboxy-terminal fragment of IQGAP1, which includes the CLIP-170 binding region, delocalizes GFP-CLIP-170 from the tips of microtubules and alters the microtubule array. Activated Rac1/Cdc42, IQGAP1, and CLIP-170 form a tripartite complex. Furthermore, expression of an IQGAP1 mutant defective in Rac1/Cdc42 binding induces multiple leading edges. These results indicate that Rac1/Cdc42 marks special cortical spots where the IQGAP1 and CLIP-170 complex is targeted, leading to a polarized microtubule array and cell polarization.
Mesh Terms:
Actins, Animals, COS Cells, Carrier Proteins, Cattle, Cell Polarity, Cell Size, Cercopithecus aethiops, Macromolecular Substances, Microscopy, Fluorescence, Microtubule-Associated Proteins, Microtubules, Neoplasm Proteins, Protein Binding, Pseudopodia, Vero Cells, cdc42 GTP-Binding Protein, rac1 GTP-Binding Protein, ras GTPase-Activating Proteins
Actins, Animals, COS Cells, Carrier Proteins, Cattle, Cell Polarity, Cell Size, Cercopithecus aethiops, Macromolecular Substances, Microscopy, Fluorescence, Microtubule-Associated Proteins, Microtubules, Neoplasm Proteins, Protein Binding, Pseudopodia, Vero Cells, cdc42 GTP-Binding Protein, rac1 GTP-Binding Protein, ras GTPase-Activating Proteins
Cell
Date: Jun. 28, 2002
PubMed ID: 12110184
View in: Pubmed Google Scholar
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