The Tre2 oncoprotein, implicated in Ewing's sarcoma, interacts with two components of the cytoskeleton.
The product of the Tre2 oncogene, structurally related to the Ypt/RabGTPase-activating proteins (Ypt/RabGAP), is involved in various human cancers, including Ewing's sarcoma. In order to identify proteins interacting with the GAP part of this protein, we performed yeast two-hybrid screening of two libraries. Two components of the cytoskeleton were thus ... identified, whose interaction with the GAP region was confirmed by GST-pulldown, co-immunoprecipitation, and colocalisation experiments. The proteins found to interact with the GAP region are the light regulatory chain of myosin II (Myl2) and LOC91256, a protein containing ankyrin repeats.
Mesh Terms:
Cytoskeletal Proteins, Cytoskeleton, Endopeptidases, Myosin Type II, Neoplasm Proteins, Oncogene Proteins, Protein Binding, Protein Interaction Mapping, Proto-Oncogene Proteins, Sarcoma, Ewing's, Two-Hybrid System Techniques, Ubiquitin Thiolesterase
Cytoskeletal Proteins, Cytoskeleton, Endopeptidases, Myosin Type II, Neoplasm Proteins, Oncogene Proteins, Protein Binding, Protein Interaction Mapping, Proto-Oncogene Proteins, Sarcoma, Ewing's, Two-Hybrid System Techniques, Ubiquitin Thiolesterase
Biotechnol. Lett.
Date: Feb. 01, 2006
PubMed ID: 16555005
View in: Pubmed Google Scholar
Download Curated Data For This Publication
71266
Switch View:
- Interactions 6