Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb.
hRPB11 is a core subunit of RNA polymerase II (pol II) specifically down-regulated on doxorubicin (dox) treatment. Levels of this protein profoundly affect cell differentiation, cell proliferation, and tumorigenicity in vivo. Here we describe Che-1, a novel human protein that interacts with hRPB11. Che-1 possesses a domain of high homology ... with Escherichia coli RNA polymerase final sigma-factor 70 and SV40 large T antigen. In addition, we report that Che-1 interacts with the retinoblastoma susceptibility gene (Rb) by two distinct domains. Functionally, we demonstrate that Che-1 represses the growth suppression function of Rb, counteracting the inhibitory action of Rb on the trans-activation function of E2F1. These results identify a novel protein that binds Rb and the core of pol II, and suggest that Che-1 may be part of transcription regulatory complex.
Mesh Terms:
Amino Acid Sequence, Carrier Proteins, Cell Cycle, Cell Cycle Proteins, Cell Division, Cell Line, Cloning, Molecular, DNA-Binding Proteins, E2F Transcription Factors, E2F1 Transcription Factor, Humans, Molecular Sequence Data, Protein Binding, RNA Polymerase II, Retinoblastoma Protein, Retinoblastoma-Binding Protein 1, Sequence Homology, Amino Acid, Transcription Factor DP1, Transcription Factors
Amino Acid Sequence, Carrier Proteins, Cell Cycle, Cell Cycle Proteins, Cell Division, Cell Line, Cloning, Molecular, DNA-Binding Proteins, E2F Transcription Factors, E2F1 Transcription Factor, Humans, Molecular Sequence Data, Protein Binding, RNA Polymerase II, Retinoblastoma Protein, Retinoblastoma-Binding Protein 1, Sequence Homology, Amino Acid, Transcription Factor DP1, Transcription Factors
FASEB J.
Date: May. 01, 2000
PubMed ID: 10783144
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