HD-PTP and Alix share some membrane-traffic related proteins that interact with their Bro1 domains or proline-rich regions.

Mammalian Alix is a multifunctional adaptor protein involved in cell death, receptor endocytosis, endosomal protein sorting and cell adhesion by associating with various proteins such as ALG-2, CIN85/Rukl/SETA, endophilins, CHMP4s and TSG101. HD-PTP is a paralog of Alix and a putative protein tyrosine phosphatase (PTP) that contains a Bro1 domain, ...
coiled-coils, a proline-rich region (PRR) in addition to a PTP domain. We investigated interactions between HD-PTP and Alix-binding proteins. In the yeast two-hybrid assay, HD-PTP showed positive interactions with CHMP4b/Shax1, TSG101, endophilin A1 and ALG-2 but not with either RabGAPLP or CIN85. We confirmed the interactions in a mammalian system by Strep-pulldown assays in which pulldown products from the lysates of HEK293T cells expressing either Strep-tagged HD-PTP alone or co-expressing with epitope-tagged proteins were analyzed by Western blotting using specific antibodies. While Alix associated with both ALG-2 and TSG101 in a Ca2+-dependent manner, HD-PTP interacted with ALG-2 Ca2+-dependently but with TSG101 Ca2+-independently.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Apoptosis Regulatory Proteins, Biological Transport, Active, Calcium, Calcium-Binding Proteins, Carrier Proteins, Cell Cycle Proteins, Cell Line, DNA-Binding Proteins, Endosomal Sorting Complexes Required for Transport, Humans, Intracellular Membranes, Membrane Transport Proteins, Proline, Protein Binding, Protein Structure, Tertiary, Protein Tyrosine Phosphatases, Protein Tyrosine Phosphatases, Non-Receptor, Transcription Factors, Two-Hybrid System Techniques
Arch. Biochem. Biophys.
Date: Jan. 15, 2007
Download Curated Data For This Publication
71721
Switch View:
  • Interactions 22