SNIP1 is a candidate modifier of the transcriptional activity of c-Myc on E box-dependent target genes.

Using a yeast two-hybrid screen, we found that SNIP1 (Smad nuclear-interacting protein 1) associates with c-Myc, a key regulator of cell proliferation and transformation. We demonstrate that SNIP1 functions as an important regulator of c-Myc activity, binding the N terminus of c-Myc through its own C terminus, and that SNIP1 ...
enhances the transcriptional activity of c-Myc both by stabilizing it against proteosomal degradation and by bridging the c-Myc/p300 complex. These effects of SNIP1 on c-Myc likely contribute to synergistic effects of SNIP1, c-Myc, and H-Ras in inducing formation of foci in an in vitro transformation assay and also in supporting anchorage-independent growth. The significant association of SNIP1 and c-Myc staining in a non-small cell lung cancer tissue array is further evidence that their activities might be linked and suggests that SNIP1 might be an important modulator of c-Myc activity in carcinogenesis.
Mesh Terms:
Carcinoma, Non-Small-Cell Lung, Cell Line, Cells, Cultured, Chromatin Immunoprecipitation, Humans, Immunohistochemistry, Intracellular Signaling Peptides and Proteins, Promoter Regions, Genetic, Proto-Oncogene Proteins c-myc, RNA, Small Interfering, Reverse Transcriptase Polymerase Chain Reaction, S-Phase Kinase-Associated Proteins, Sensitivity and Specificity, Tissue Array Analysis, Transcription, Genetic, Two-Hybrid System Techniques
Mol. Cell
Date: Dec. 08, 2006
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