Ribosome-associated protein LBP/p40 binds to S21 protein of 40S ribosome: analysis using a yeast two-hybrid system.

The ribosome-associated protein LBP/p40, which was originally named after "laminin binding protein precursor p40," is distributed on the cell surface as laminin binding protein p67 (LBP/p67), in the nucleus, and on 40S ribosomes. In a broad range of eukaryotes, the localization of LBP/p40 on the 40S ribosome is well conserved. ...
Two yeast homologs of LBP/p40 are believed to be essential for cell viability and each gene product probably corresponds to the assembly and/or stability of the 40S ribosomal subunit. The precise role of LBP/p40 in translation, however, remains to be elucidated, especially in higher eukaryotes. In this report, we used a yeast two-hybrid screening method to isolate molecules associated with human LBP/p40 protein on ribosomes. We found that the 40S ribosomal protein S21 was tightly bound with LBP/p40 in this yeast two-hybrid system and in in vitro analysis. Further, we discovered that the association required a broad region of the LBP/p40 amino acid sequence, which corresponds to the highly conserved region of LBP/p40 homologs among eukaryotes.
Mesh Terms:
5' Untranslated Regions, Amino Acid Sequence, Binding Sites, Blotting, Western, Cell Division, Conserved Sequence, False Positive Reactions, Gene Library, Genes, Reporter, Hela Cells, Humans, Open Reading Frames, Promoter Regions, Genetic, Protein Binding, Protein Precursors, Receptors, Laminin, Recombinant Fusion Proteins, Ribosomal Proteins, Saccharomyces cerevisiae, Sequence Deletion
Biochem. Biophys. Res. Commun.
Date: Mar. 16, 1999
Download Curated Data For This Publication
7223
Switch View:
  • Interactions 1