KIAA0649, a 1A6/DRIM-interacting protein with the oncogenic potential.
We identified a 1A6/DRIM (down-regulated in metastasis) interacting protein, KIAA0649 during the yeast two-hybrid screen. The interaction between KIAA0649 and 1A6/DRIM was further confirmed by GST-pull-down and co-immunoprecipitation assays. KIAA0649 was originally identified from human brain tissue. However, its biological function remains unknown. In this report, we showed that KIAA0649 ... mRNA is widely expressed in human multiple tissues and cell lines. We have also demonstrated that KIAA0649 has oncogenic characteristics: it enhances colony formation, allows anchorage-independent growth. Moreover, KIAA0649 exogenous expression in NIH3T3 fibroblasts caused tumor development in nude mice.
Mesh Terms:
Animals, COS Cells, Cell Line, Tumor, Cell Proliferation, Cell Transformation, Neoplastic, Cercopithecus aethiops, Female, Hela Cells, Humans, Male, Mice, Mice, Nude, NIH 3T3 Cells, Nerve Tissue Proteins, Nuclear Proteins, Oncogene Proteins, Proteins, Ribonucleoproteins, Two-Hybrid System Techniques
Animals, COS Cells, Cell Line, Tumor, Cell Proliferation, Cell Transformation, Neoplastic, Cercopithecus aethiops, Female, Hela Cells, Humans, Male, Mice, Mice, Nude, NIH 3T3 Cells, Nerve Tissue Proteins, Nuclear Proteins, Oncogene Proteins, Proteins, Ribonucleoproteins, Two-Hybrid System Techniques
Biochem. Biophys. Res. Commun.
Date: Sep. 02, 2005
PubMed ID: 16053918
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