The PEF family proteins sorcin and grancalcin interact in vivo and in vitro.
The penta-EF hand (PEF) family of calcium binding proteins includes grancalcin, peflin, sorcin, calpain large and small subunits as well as ALG-2. Systematic testing of the heterodimerization abilities of the PEF proteins using the yeast two-hybrid and glutathione S-transferase pull-down assays revealed the new finding that grancalcin interacts strongly with ... sorcin. In addition, sorcin and grancalcin can be co-immunoprecipitated from lysates of human umbilical vein endothelial cells. Our results indicate that heterodimerization, in addition to differential interactions with target proteins, might be a way to regulate and fine tune processes mediated by calcium binding proteins of the penta-EF hand type.
Mesh Terms:
Calcium-Binding Proteins, Cells, Cultured, Dimerization, EF Hand Motifs, Endothelium, Vascular, Glutathione Transferase, Humans, Precipitin Tests, Recombinant Fusion Proteins, Two-Hybrid System Techniques, Umbilical Veins
Calcium-Binding Proteins, Cells, Cultured, Dimerization, EF Hand Motifs, Endothelium, Vascular, Glutathione Transferase, Humans, Precipitin Tests, Recombinant Fusion Proteins, Two-Hybrid System Techniques, Umbilical Veins
FEBS Lett.
Date: Jun. 19, 2003
PubMed ID: 12804766
View in: Pubmed Google Scholar
Download Curated Data For This Publication
7328
Switch View:
- Interactions 4