ATM binds to beta-adaptin in cytoplasmic vesicles.

Inherited mutations in the ATM gene lead to a complex clinical phenotype characterized by neuronal degeneration, oculocutaneous telangiectasias, immune dysfunction, and cancer predisposition. Using the yeast two-hybrid system, we demonstrate that ataxia telangiectasia mutated (ATM) binds to beta-adaptin, one of the components of the AP-2 adaptor complex, which is involved ...
in clathrin-mediated endocytosis of receptors. The interaction between ATM and beta-adaptin was confirmed in vitro, and coimmunoprecipitation and colocalization studies show that the proteins also associate in vivo. ATM also interacts in vitro with beta-NAP, a neuronal-specific beta-adaptin homolog that was identified as an autoantigen in a patient with cerebellar degeneration. Our data describing the association of ATM with beta-adaptin in vesicles indicate that ATM may play a role in intracellular vesicle and/or protein transport mechanisms.
Mesh Terms:
Adaptor Protein Complex 3, Adaptor Protein Complex beta Subunits, Animals, Ataxia Telangiectasia, Autoantigens, Base Sequence, Cell Cycle Proteins, Cercopithecus aethiops, Cerebellum, Cytoplasm, DNA Primers, DNA-Binding Proteins, Humans, Membrane Proteins, Mice, Nerve Tissue Proteins, Peptide Fragments, Phosphoproteins, Protein Binding, Protein-Serine-Threonine Kinases, Proteins, Saccharomyces cerevisiae, Transfection, Tumor Suppressor Proteins, Vero Cells
Proc. Natl. Acad. Sci. U.S.A.
Date: Aug. 18, 1998
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