Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice.
When replication forks collapse, Rad3 phosphorylates the checkpoint-clamp protein Rad9 in a manner that depends on Thr 225, a residue within the PCNA-like domain. The physiological function of Thr 225-dependent Rad9 phosphorylation, however, remains elusive. Here, we show that Thr 225-dependent Rad9 phosphorylation by Rad3 regulates DNA repair pathways. A ... rad9(T225C) mutant induces a translesion synthesis (TLS)-dependent high spontaneous mutation rate and a hyper-recombination phenotype. Consistent with this, Rad9 coprecipitates with the post-replication repair protein Mms2. This interaction is dependent on Rad9 Thr 225 and is enhanced by DNA damage. Genetic analyses indicate that Thr 225-dependent Rad9 phosphorylation prevents inappropriate Rhp51-dependent recombination, potentially by redirecting the repair through a Pli1-mediated sumoylation pathway into the error-free branch of the Rhp6 repair pathway. Our findings reveal a new mechanism by which phosphorylation of Rad9 at Thr 225 regulates the choice of repair pathways for maintaining genomic integrity during the cell cycle.
Mesh Terms:
Cell Cycle, Cell Cycle Proteins, DNA Damage, DNA Repair, Gene Expression Regulation, Fungal, Genes, cdc, Genomic Instability, Phosphorylation, Protein Kinases, Saccharomyces cerevisiae, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction, Threonine, Ubiquitin-Conjugating Enzymes
Cell Cycle, Cell Cycle Proteins, DNA Damage, DNA Repair, Gene Expression Regulation, Fungal, Genes, cdc, Genomic Instability, Phosphorylation, Protein Kinases, Saccharomyces cerevisiae, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction, Threonine, Ubiquitin-Conjugating Enzymes
Nat. Cell Biol.
Date: Jun. 01, 2007
PubMed ID: 17515930
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