BRCA1 supports XIST RNA concentration on the inactive X chromosome.
BRCA1, a breast and ovarian tumor suppressor, colocalizes with markers of the inactive X chromosome (Xi) on Xi in female somatic cells and associates with XIST RNA, as detected by chromatin immunoprecipitation. Breast and ovarian carcinoma cells lacking BRCA1 show evidence of defects in Xi chromatin structure. Reconstitution of BRCA1-deficient ... cells with wt BRCA1 led to the appearance of focal XIST RNA staining without altering XIST abundance. Inhibiting BRCA1 synthesis in a suitable reporter line led to increased expression of an otherwise silenced Xi-located GFP transgene. These observations suggest that loss of BRCA1 in female cells may lead to Xi perturbation and destabilization of its silenced state.
Mesh Terms:
Animals, BRCA1 Protein, Carrier Proteins, DNA Methylation, Dosage Compensation, Genetic, Female, Gene Expression, Histones, Humans, Lysine, Male, Mice, RNA, RNA, Untranslated, RNA-Binding Proteins, Spermatocytes, Staining and Labeling, Transcription Factors, Tumor Cells, Cultured, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, X Chromosome
Animals, BRCA1 Protein, Carrier Proteins, DNA Methylation, Dosage Compensation, Genetic, Female, Gene Expression, Histones, Humans, Lysine, Male, Mice, RNA, RNA, Untranslated, RNA-Binding Proteins, Spermatocytes, Staining and Labeling, Transcription Factors, Tumor Cells, Cultured, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, X Chromosome
Cell
Date: Nov. 01, 2002
PubMed ID: 12419249
View in: Pubmed Google Scholar
Download Curated Data For This Publication
73994
Switch View:
- Interactions 5