LDL receptor-related protein as a component of the midkine receptor.
Midkine (MK) is a heparin-binding growth factor with migration-promoting and survival-promoting activities. To identify signaling receptor(s) of MK, membrane glycoproteins with MK-binding activity were isolated from day 13 mouse embryos by lectin- and MK-affinity chromatography. SDS-PAGE followed by protein sequence analysis revealed the presence of LDL receptor-related protein (LRP) and ... NCAM in the fraction. The dissociation constant of binding between LRP and MK was 3.5 nM. Receptor-associated protein (RAP), which interfered with the binding, inhibited MK-dependent survival of embryonic neurons. Brushin/megalin, which is also a high molecular weight protein belonging to the LDL receptor family, bound to MK less strongly than LRP. These findings suggest that LRP is a component of the receptor complex for MK.
Mesh Terms:
Amino Acid Sequence, Animals, Carrier Proteins, Cytokines, Embryo, Mammalian, Heymann Nephritis Antigenic Complex, Humans, Kinetics, LDL-Receptor Related Protein 1, LDL-Receptor Related Protein 2, Membrane Glycoproteins, Mice, Molecular Sequence Data, Nerve Growth Factors, Neural Cell Adhesion Molecules, Receptors, Cytokine, Receptors, Growth Factor, Receptors, Immunologic, Recombinant Proteins, Signal Transduction
Amino Acid Sequence, Animals, Carrier Proteins, Cytokines, Embryo, Mammalian, Heymann Nephritis Antigenic Complex, Humans, Kinetics, LDL-Receptor Related Protein 1, LDL-Receptor Related Protein 2, Membrane Glycoproteins, Mice, Molecular Sequence Data, Nerve Growth Factors, Neural Cell Adhesion Molecules, Receptors, Cytokine, Receptors, Growth Factor, Receptors, Immunologic, Recombinant Proteins, Signal Transduction
Biochem. Biophys. Res. Commun.
Date: Apr. 21, 2000
PubMed ID: 10772929
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