Expression and interaction of different catenins in colorectal carcinoma cells.

Aberrant signalling activities of beta-catenin, originally identified as a component of cell-adhesion complexes, are now considered to be an important factor in colorectal carcinogenesis. However, recently it was shown that also gamma- as well as p120 catenins have a dual role either in cell adhesion or in affecting some gene ...
activation. Therefore, the levels and interactions of these three catenins in human colorectal carcinoma cell lines were analysed. A great heterogeneity in the expression of all catenins tested was found in colorectal carcinoma cell lines HT29 and LS174T. Detailed analysis of beta-catenin interactions was done. GST-APC fragment-fused proteins were used to absorb beta-catenin and its complexes from cell lysates. Similarly, the E-cadherin binding capacity of the residual pool of beta-catenin was analysed using the GST-ECT construct. It was found that the level of beta-catenin does not necessarily depend either on the APC or beta-catenin gene mutations and that co-precipitation of beta-, gamma-, and p120 catenins is not limited to cells that express E-cadherin.
Mesh Terms:
Binding, Competitive, Blotting, Western, Cadherins, Cell Adhesion Molecules, Colorectal Neoplasms, Cytoskeletal Proteins, Desmoplakins, HT29 Cells, Humans, Phosphoproteins, Precipitin Tests, Protein Binding, Trans-Activators, Tumor Cells, Cultured, beta Catenin
Int. J. Mol. Med.
Date: Dec. 01, 2001
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