BCR sequences essential for transformation by the BCR-ABL oncogene bind to the ABL SH2 regulatory domain in a non-phosphotyrosine-dependent manner.

BCR-ABL is a chimeric oncogene implicated in the pathogenesis of Philadelphia chromosome-positive human leukemias. BCR first exon sequences specifically activate the tyrosine kinase and transforming potential of BCR-ABL. We have tested the hypothesis that activation of BCR-ABL may involve direct interaction between BCR sequences and the tyrosine kinase regulatory domains ...
of ABL. Full-length c-BCR as well as BCR sequences retained in BCR-ABL bind specifically to the SH2 domain of ABL. The binding domain has been localized within the first exon of BCR and consists of at least two SH2-binding sites. This domain is essential for BCR-ABL-mediated transformation. Phosphoserine/phosphothreonine but not phosphotyrosine residues on BCR are required for interaction with the ABL SH2 domain. These findings extend the range of potential SH2-protein interactions in growth control pathways and suggest a function for SH2 domains in the activation of the BCR-ABL oncogene as well as a role for BCR in cellular signaling pathways.
Mesh Terms:
Amino Acid Sequence, Animals, Binding Sites, Cell Line, Cell Transformation, Neoplastic, Exons, Fusion Proteins, bcr-abl, Genes, abl, Genetic Variation, Humans, Insects, Molecular Sequence Data, Oncogene Proteins, Oncogenes, Phosphotyrosine, Plasmids, Protein Biosynthesis, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcr, Regulatory Sequences, Nucleic Acid, Transcription, Genetic, Transfection, Tyrosine
Cell
Date: Jul. 12, 1991
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