Genetic analysis of BRCA1 function in a defined tumor cell line.
Retrovirally expressed, wild-type BRCA1 decreased the gamma radiation (IR) sensitivity and increased the efficiency of double-strand DNA break repair (DSBR) of the BRCA1-/- human breast cancer line, HCC1937. It also reduced its susceptibility to DSB generation by IR. By contrast, multiple, clinically validated, missense mutant BRCA1 products were nonfunctional in ... these assays. These data constitute the basis for a BRCA1 functional assay and suggest that efficient repair of double-strand DNA breaks is linked to BRCA1 tumor suppression function.
Mesh Terms:
BRCA1 Protein, Breast Neoplasms, DNA Repair, Female, Gene Expression Regulation, Neoplastic, Gene Transfer Techniques, Genetic Vectors, Humans, Radiation Tolerance, Retroviridae, Tumor Cells, Cultured
BRCA1 Protein, Breast Neoplasms, DNA Repair, Female, Gene Expression Regulation, Neoplastic, Gene Transfer Techniques, Genetic Vectors, Humans, Radiation Tolerance, Retroviridae, Tumor Cells, Cultured
Mol. Cell
Date: Dec. 01, 1999
PubMed ID: 10635334
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