Human stoned B interacts with AP-2 and synaptotagmin and facilitates clathrin-coated vesicle uncoating.

Synaptic vesicle biogenesis involves the recycling of synaptic vesicle components by clathrin-mediated endocytosis from the presynaptic membrane. stoned B, a protein encoded by the stoned locus in Drosophila melanogaster has been shown to regulate vesicle recycling by interacting with synaptotagmin. We report here the identification and characterization of a human ...
homolog of stoned B (hStnB). Human stoned B is a brain-specific protein which co-enriches with other endocytic proteins such as AP-2 in a crude synaptic vesicle fraction and at nerve terminals. A domain with homology to the medium chain of adaptor complexes binds directly to both AP-2 and synaptotagmin and competes with AP-2 for the same binding site within synaptotagmin. Finally we show that the mu 2 homology domain of hStnB stimulates the uncoating of both clathrin and AP-2 adaptors from clathrin-coated vesicles. We hypothesize that hStnB regulates synaptic vesicle recycling by facilitating vesicle uncoating.
Mesh Terms:
Adaptor Proteins, Vesicular Transport, Amino Acid Sequence, Animals, Binding Sites, Brain Chemistry, Calcium-Binding Proteins, Carrier Proteins, Cell Fractionation, Clathrin-Coated Vesicles, Drosophila, Drosophila Proteins, Enzyme Inhibitors, Genes, Reporter, Humans, Immunoblotting, Membrane Glycoproteins, Membrane Proteins, Microscopy, Fluorescence, Molecular Sequence Data, Nerve Tissue Proteins, Protein Binding, Protein Structure, Tertiary, Rats, Recombinant Fusion Proteins, Sequence Alignment, Synaptotagmins, Tissue Distribution
EMBO Rep.
Date: Jul. 01, 2001
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