Identification of a conserved motif required for Vps35p/Vps26p interaction and assembly of the retromer complex.
The retromer complex is a conserved cytoplasmic coat complex that mediates the endosome-to-Golgi retrieval of vacuole/lysosome hydrolase receptors in yeast and mammals. The recognition of cargo proteins by the retromer is performed by the Vps35p/VPS35 (where Vps is vacuolar protein sorting) component, which together with Vps26p/VPS26 and Vps29p/VPS29, forms the ... cargo-selective subcomplex. In this report, we have identified a highly-conserved region of Vps35p/VPS35 that is essential for the interaction with Vps26p/VPS26 and for assembly of the retromer complex. Mutation of residues within the conserved region results in Vps35p/VPS35 mutants, which cannot bind to Vps26p/VPS26 and are not efficiently targeted to the endosomal membrane. These data implicate Vps26p/VPS26 in regulating Vps35p/VPS35 membrane association and therefore suggest a role for Vps26p/VPS26 in cargo recognition.
Mesh Terms:
Amino Acid Motifs, Amino Acid Sequence, Cell Membrane, Conserved Sequence, Cytoplasm, Hela Cells, Humans, Molecular Sequence Data, Mutation, Protein Transport, Vesicular Transport Proteins
Amino Acid Motifs, Amino Acid Sequence, Cell Membrane, Conserved Sequence, Cytoplasm, Hela Cells, Humans, Molecular Sequence Data, Mutation, Protein Transport, Vesicular Transport Proteins
Biochem. J.
Date: Dec. 01, 2007
PubMed ID: 17696874
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