tRNA import into yeast mitochondria is regulated by the ubiquitin-proteasome system.
In Saccharomyces cerevisiae, one of two cytosolic lysine-tRNAs is partially imported into mitochondria. We demonstrate that three components of the ubiquitin/26S proteasome system (UPS), Rpn13p, Rpn8p and Doa1p interact with the imported tRNA and with the essential factor of its mitochondrial targeting, pre-Msk1p. Genetic and biochemical assays demonstrate that UPS ... plays a dual regulatory role, since the overall inhibition of cellular proteasome activity reduces tRNA import, while specific depletion of Rpn13p or Doa1p increases it. This result suggests a functional link between UPS and tRNA mitochondrial import in yeast and indicates on the existence of negative and positive import regulators.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Carrier Proteins, Mitochondria, Models, Biological, Mutation, Phenotype, Proteasome Endopeptidase Complex, Protein Binding, Protein Precursors, Protein Processing, Post-Translational, RNA Transport, RNA, Fungal, RNA, Transfer, Ribosomal Protein S6 Kinases, 90-kDa, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Ubiquitin
Adaptor Proteins, Signal Transducing, Carrier Proteins, Mitochondria, Models, Biological, Mutation, Phenotype, Proteasome Endopeptidase Complex, Protein Binding, Protein Precursors, Protein Processing, Post-Translational, RNA Transport, RNA, Fungal, RNA, Transfer, Ribosomal Protein S6 Kinases, 90-kDa, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Ubiquitin
FEBS Lett.
Date: Sep. 04, 2007
PubMed ID: 17707817
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