Regulation of Saccharomyces cerevisiae DNA polymerase eta transcript and protein.

RAD30-encoded DNA polymerase eta functions as a translesion polymerase that can bypass the most frequent types of UV-induced pyrimidine photoproducts in an error-free manner. Although its transcript is UV-inducible in Saccharomyces cerevisiae, Rad30 (studied as a Rad30-Myc fusion) is a stable protein whose levels do not fluctuate following UV treatment ...
or during cell cycle progression. Rad30 protein is subject to monoubiquitination whose level is upregulated in G1 and downregulated during S-phase reentry. This downregulation is accelerated in UV-treated cells. A missense mutation (L577Q) of the ubiquitin binding domain (UBZ) confers a reduced degree of ubiquitination outside of G1 and a complete failure to stably interact with ubiquitinated substrates. This mutation confers a phenotype resembling a complete RAD30 deletion, thus attesting to the significance of the UBZ motif for polymerase eta function in vivo.
Mesh Terms:
Amino Acid Motifs, DNA Damage, DNA Repair, DNA Replication, DNA-Directed DNA Polymerase, Enzyme Activators, Genes, Fungal, RNA, Fungal, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Ubiquitin-Conjugating Enzymes, Ubiquitination, Ultraviolet Rays
Radiat Environ Biophys
Date: Feb. 01, 2008
74370
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