Tomosyn: a syntaxin-1-binding protein that forms a novel complex in the neurotransmitter release process.

Syntaxin-1 is a component of the synaptic vesicle docking and/or membrane fusion soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) complex (7S and 20S complexes) in nerve terminals. Syntaxin-1 also forms a heterodimer with Munc18/n-Sec1/rbSec1 in a complex that is distinct from the 7S and 20S complexes. In this report, we identify ...
a novel syntaxin-1-binding protein, tomosyn, that is capable of dissociating Munc18 from syntaxin-1 and forming a novel 10S complex with syntaxin-1, soluble N-etyhlmaleimide-sensitive factor attachment (SNAP) 25, and synaptotagmin. The 130 kDa isoform of tomosyn is specifically expressed in brain, where its distribution partly overlaps with that of syntaxin-1 in nerve terminals. High level expression of either syntaxin-1 or tomosyn results in a specific reduction in Ca2+-dependent exocytosis from PC12 cells. These results suggest that tomosyn is an important component in the neurotransmitter release process where it may stimulate SNARE complex formation.
Mesh Terms:
Animals, Antigens, Surface, Blotting, Western, Brain Chemistry, COS Cells, Calcium, Carrier Proteins, Cloning, Molecular, DNA, Complementary, Exocytosis, Isomerism, Molecular Sequence Data, Munc18 Proteins, Nerve Tissue Proteins, Neurons, Neuropeptides, Neurotransmitter Agents, PC12 Cells, Protein Binding, R-SNARE Proteins, Rats, Synaptic Transmission, Syntaxin 1, Vesicular Transport Proteins
Neuron
Date: May. 01, 1998
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