Peroxiredoxin-4 interacts with and regulates the thromboxane A(2) receptor.
We identified peroxiredoxin-4 (Prx-4) as a protein interacting with the beta isoform of the thromboxane A(2) receptor (TPbeta) by yeast two-hybrid analysis. Prx-4 co-immunoprecipitated constitutively with TPbeta in HEK293 cells. The second and third intracellular loops as well as the C-terminus of TPbeta interacted directly with Prx-4. Co-expression of Prx-4 ... caused a 60% decrease in cell surface expression of TPbeta. Prx-4 and TPbeta predominantly co-localized in the endoplasmic reticulum. Co-expression of Prx-4 in cells treated with H(2)O(2) targeted TPbeta for degradation. We show for the first time an interaction between a receptor involved in oxidative stress and Prx-4, an anti-oxidative enzyme.
Mesh Terms:
Cell Line, Endoplasmic Reticulum, Fluorescent Antibody Technique, Indirect, Gene Expression Regulation, Green Fluorescent Proteins, Humans, Hydrogen Peroxide, Inositol Phosphates, Oxidants, Oxidative Stress, Peroxidases, Peroxiredoxins, Precipitin Tests, Protein Isoforms, Protein Transport, Receptors, Thromboxane A2, Prostaglandin H2, Recombinant Fusion Proteins, Signal Transduction, Transfection, Two-Hybrid System Techniques
Cell Line, Endoplasmic Reticulum, Fluorescent Antibody Technique, Indirect, Gene Expression Regulation, Green Fluorescent Proteins, Humans, Hydrogen Peroxide, Inositol Phosphates, Oxidants, Oxidative Stress, Peroxidases, Peroxiredoxins, Precipitin Tests, Protein Isoforms, Protein Transport, Receptors, Thromboxane A2, Prostaglandin H2, Recombinant Fusion Proteins, Signal Transduction, Transfection, Two-Hybrid System Techniques
FEBS Lett.
Date: Aug. 07, 2007
PubMed ID: 17644091
View in: Pubmed Google Scholar
Download Curated Data For This Publication
74956
Switch View:
- Interactions 3