Identification of flotillin-1 as an interacting protein for antisecretory factor.
Antisecretory factor (AF) also named S5a/Rpn10 was originally identified through its capacity to inhibit intestinal hypersecretion and was later shown to be a component in the proteasome complex. AF is also a potent anti-inflammatory agent and can act as a neuromodulator. In this study we used yeast two-hybrid screens, with ... yeast strain PJ692A transformed with the bait vector pGBKT7 (AF aa 1-105) against yeast strain Y187 pretransformed with human brain or placenta cDNA libraries, to identify AF-binding proteins. Flotillin-1 was identified as a specific interacting factor with AF. Immunohistochemistry showed co-localization of AF and flotillin-1 in nervous tissue. Flotillin-1 is an integral membrane protein and a component of lipid rafts, a membrane specialization involved in transport processes. Intracellular AF may affect secretory processes by regulating the localization of signal proteins to lipid rafts.
Mesh Terms:
Cells, Cultured, Computer Simulation, Humans, Membrane Proteins, Models, Molecular, Neuropeptides, Protein Binding, Two-Hybrid System Techniques, Yeasts
Cells, Cultured, Computer Simulation, Humans, Membrane Proteins, Models, Molecular, Neuropeptides, Protein Binding, Two-Hybrid System Techniques, Yeasts
Regul. Pept.
Date: Feb. 07, 2008
PubMed ID: 18164080
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