Cyclophilin A and FKBP12 interact with YY1 and alter its transcriptional activity.
YY1 is a zinc finger transcription factor with unusual structural and functional features. In a yeast two-hybrid screen, two cellular proteins, cyclophilin A (CyPA) and FK506-binding protein 12 (FKBP12), interacted with YY1. These interactions are specific and also occur in mammalian cells. Cyclosporin A and FK506 efficiently disrupt the YY1-CyPA ... and YY1-FKBP12 interactions. Overexpression of human CyPA and FKBP12 have different effects on YY1-regulated transcription with these effects being promoter-dependent. These results suggest that immunophilins may be mediators in the functional role of YY1.
Mesh Terms:
Amino Acid Isomerases, Carrier Proteins, Chloramphenicol O-Acetyltransferase, Cyclosporine, DNA-Binding Proteins, Endodeoxyribonucleases, Erythroid-Specific DNA-Binding Factors, Heat-Shock Proteins, Hela Cells, Humans, Peptidylprolyl Isomerase, Promoter Regions, Genetic, Recombinant Fusion Proteins, TATA Box, Tacrolimus, Tacrolimus Binding Proteins, Transcription Factors, Transcription, Genetic, Transfection, YY1 Transcription Factor, Zinc Fingers
Amino Acid Isomerases, Carrier Proteins, Chloramphenicol O-Acetyltransferase, Cyclosporine, DNA-Binding Proteins, Endodeoxyribonucleases, Erythroid-Specific DNA-Binding Factors, Heat-Shock Proteins, Hela Cells, Humans, Peptidylprolyl Isomerase, Promoter Regions, Genetic, Recombinant Fusion Proteins, TATA Box, Tacrolimus, Tacrolimus Binding Proteins, Transcription Factors, Transcription, Genetic, Transfection, YY1 Transcription Factor, Zinc Fingers
J. Biol. Chem.
Date: Jun. 23, 1995
PubMed ID: 7541038
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