Histone chaperones regulate histone exchange during transcription.
Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, ... using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.
Mesh Terms:
Cell Cycle Proteins, Fungal Proteins, Gene Deletion, Gene Silencing, Histones, Molecular Chaperones, Mutation, Nuclear Proteins, Nucleosomes, RNA Polymerase II, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription, Genetic
Cell Cycle Proteins, Fungal Proteins, Gene Deletion, Gene Silencing, Histones, Molecular Chaperones, Mutation, Nuclear Proteins, Nucleosomes, RNA Polymerase II, Repressor Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription, Genetic
EMBO J.
Date: Oct. 31, 2007
PubMed ID: 17914459
View in: Pubmed Google Scholar
Download Curated Data For This Publication
75895
Switch View:
- Interactions 4