Identification of a fatty acyl-CoA synthetase gene, lcf2+, which affects viability after entry into the stationary phase in Schizosaccharomyces pombe.
The lcf1(+) gene, which encodes a long chain fatty acyl-CoA synthetase, is necessary for the maintenance of viability after entry into the stationary phase in Schizosaccharomyces pombe. In this study, we analyzed a paralogous gene, SPBP4H10.11c (named lcf2(+)), and we present evidence that the gene encodes a new fatty acyl-CoA ... synthetase. The enzyme preferentially recognized myristic acid as a substrate. A Deltalcf2 mutant showed increased viability after entry into the stationary phase in SD medium. A Deltalcf1Deltalcf2 double mutant showed a severe decrease in long-chain fatty acyl-CoA synthetase activity and a rapid loss of viability after entry into the stationary phase. These results suggest that fatty acid utilization and/or metabolism is important to determine viability in the stationary phase.
Mesh Terms:
Acyl Coenzyme A, Amino Acid Sequence, Microbial Viability, Molecular Sequence Data, Mutation, Myristic Acid, Schizosaccharomyces, Substrate Specificity
Acyl Coenzyme A, Amino Acid Sequence, Microbial Viability, Molecular Sequence Data, Mutation, Myristic Acid, Schizosaccharomyces, Substrate Specificity
Biosci. Biotechnol. Biochem.
Date: Dec. 01, 2007
PubMed ID: 18071249
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