Human MCRS2, a cell-cycle-dependent protein, associates with LPTS/PinX1 and reduces the telomere length.
Human LPTS/PinX1 is a telomerase-inhibitory protein, which binds to the telomere protein Pin2/TRF1 and the catalytic subunit hTERT of telomerase. To explore the proteins that might be involved in the telomerase pathway, we performed a yeast two-hybrid screening with LPTS/PinX1 as the bait. A novel gene, MCRS2, encoding for an ... isoform of MCRS1/p78 and MSP58 was isolated. The expression of MCRS2 protein is cell-cycle dependent, accumulating in the very early S phase. MCRS2 interacts with LPTS/PinX1 in vitro, in vivo and colocalizes with LPTS/PinX1 in cells. MCRS2 and its amino terminus inhibit telomerase activity in vitro and long-term overexpression of MCRS2 in SMMC-7721 cells results in a gradual and progressive shortening of telomeres. Our findings suggest that MCRS2 might be a linker between telomere maintenance and cell-cycle regulation.
Mesh Terms:
Base Sequence, Brain, Carcinoma, Hepatocellular, Cell Cycle, Cell Cycle Proteins, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Humans, Liver, Molecular Sequence Data, Nuclear Proteins, Proteins, RNA-Binding Proteins, Telomere, Tumor Suppressor Proteins
Base Sequence, Brain, Carcinoma, Hepatocellular, Cell Cycle, Cell Cycle Proteins, Cell Line, Tumor, Gene Expression Regulation, Neoplastic, Humans, Liver, Molecular Sequence Data, Nuclear Proteins, Proteins, RNA-Binding Proteins, Telomere, Tumor Suppressor Proteins
Biochem. Biophys. Res. Commun.
Date: Apr. 16, 2004
PubMed ID: 15044100
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