FKF1 and GIGANTEA complex formation is required for day-length measurement in Arabidopsis.
Precise timing of CONSTANS (CO) gene expression is necessary for day-length discrimination for photoperiodic flowering. The FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1), and GIGANTEA (GI) proteins regulate CO transcription in Arabidopsis. We demonstrate that FKF1 and GI proteins form a complex in a blue-light-dependent manner. The timing of this interaction ... regulates the timing of daytime CO expression. FKF1 function is dependent on GI, which interacts with a CO repressor, CYCLING DOF FACTOR 1 (CDF1), and controls CDF1 stability. GI, FKF1, and CDF1 proteins associate with CO chromatin. Thus, the FKF1-GI complex forms on the CO promoter in late afternoon to regulate CO expression, providing a mechanistic view of how the coincidence of light with circadian timing regulates photoperiodic flowering.
Mesh Terms:
Arabidopsis, Arabidopsis Proteins, Chromatin, Chromatin Immunoprecipitation, Circadian Rhythm, DNA-Binding Proteins, Flowers, Gene Expression Regulation, Plant, Light, Mutation, Photoperiod, Plants, Genetically Modified, Promoter Regions, Genetic, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repressor Proteins, Transcription Factors
Arabidopsis, Arabidopsis Proteins, Chromatin, Chromatin Immunoprecipitation, Circadian Rhythm, DNA-Binding Proteins, Flowers, Gene Expression Regulation, Plant, Light, Mutation, Photoperiod, Plants, Genetically Modified, Promoter Regions, Genetic, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repressor Proteins, Transcription Factors
Science
Date: Oct. 12, 2007
PubMed ID: 17872410
View in: Pubmed Google Scholar
Download Curated Data For This Publication
76066
Switch View:
- Interactions 5