Direct targeting of light signals to a promoter element-bound transcription factor.
Light signals perceived by the phytochrome family of sensory photoreceptors are transduced to photoresponsive genes by an unknown mechanism. Here, we show that the basic helix-loop-helix transcription factor PIF3 binds specifically to a G-box DNA-sequence motif present in various light-regulated gene promoters, and that phytochrome B binds reversibly to G-box-bound ... PIF3 specifically upon light-triggered conversion of the photoreceptor to its biologically active conformer. We suggest that the phytochromes may function as integral light-switchable components of transcriptional regulator complexes, permitting continuous and immediate sensing of changes in this environmental signal directly at target gene promoters.
Mesh Terms:
Arabidopsis, Arabidopsis Proteins, Basic Helix-Loop-Helix Transcription Factors, Cell Cycle Proteins, DNA, Plant, DNA-Binding Proteins, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Helix-Loop-Helix Motifs, Light, Models, Genetic, Oligodeoxyribonucleotides, Photoreceptor Cells, Photosynthetic Reaction Center Complex Proteins, Phytochrome, Phytochrome B, Plant Proteins, Promoter Regions, Genetic, Recombinant Fusion Proteins, Signal Transduction, Transcription Factors
Arabidopsis, Arabidopsis Proteins, Basic Helix-Loop-Helix Transcription Factors, Cell Cycle Proteins, DNA, Plant, DNA-Binding Proteins, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Helix-Loop-Helix Motifs, Light, Models, Genetic, Oligodeoxyribonucleotides, Photoreceptor Cells, Photosynthetic Reaction Center Complex Proteins, Phytochrome, Phytochrome B, Plant Proteins, Promoter Regions, Genetic, Recombinant Fusion Proteins, Signal Transduction, Transcription Factors
Science
Date: May. 05, 2000
PubMed ID: 10797009
View in: Pubmed Google Scholar
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