LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction.

Lamina-associated polypeptide (LAP) 2, which directly interacts with B-type lamins and chromosomes, is an integral membrane protein specifically distributed along the inner nuclear membrane of the nuclear envelope. The chromatin- and lamin-binding activity of LAP2 suggests that LAP2 plays an important role in targeting mitotic vesicles to chromosomes and reorganizing ...
the nuclear structure at the end of mitosis. Here I identified a LAP2 interacting protein, termed L2BP1 (LAP2 binding protein 1). The rat L2BP1 cDNA sequence is predicted to encode a protein of 89 amino acids which turns out to be a rat homolog of mouse and human BAF (Barrier-to-Autointegration Factor). L2BP1 is distributed diffusely throughout the nucleus in interphase cells. It is, however, highly concentrated at the chromosomes during the M-phase. Further, the L2BP1 binding domain of LAP2 overlaps its chromosome-binding region. These findings suggest that L2BP1 is a candidate mediator of LAP2-chromosome interaction at the end of mitosis.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cell Line, Cell Nucleus, Chromatin, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Humans, Membrane Proteins, Mice, Molecular Sequence Data, Nuclear Proteins, Rats, Restriction Mapping, Saccharomyces cerevisiae, Sequence Alignment, Sequence Homology, Amino Acid
J. Cell. Sci.
Date: Aug. 01, 1999
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