Arabidopsis UEV1D promotes Lysine-63-linked polyubiquitination and is involved in DNA damage response.
DNA damage tolerance (DDT) in budding yeast requires Lys-63-linked polyubiquitination of the proliferating cell nuclear antigen. The ubiquitin-conjugating enzyme Ubc13 and the Ubc enzyme variant (Uev) methyl methanesulfonate2 (Mms2) are required for this process. Mms2 homologs have been found in all eukaryotic genomes examined; however, their roles in multicellular eukaryotes ... have not been elucidated. We report the isolation and characterization of four UEV1 genes from Arabidopsis thaliana. All four Uev1 proteins can form a stable complex with At Ubc13 or with Ubc13 from yeast or human and can promote Ubc13-mediated Lys-63 polyubiquitination. All four Uev1 proteins can replace yeast MMS2 DDT functions in vivo. Although these genes are ubiquitously expressed in most tissues, UEV1D appears to express at a much higher level in germinating seeds and in pollen. We obtained and characterized two uev1d null mutant T-DNA insertion lines. Compared with wild-type plants, seeds from uev1d null plants germinated poorly when treated with a DNA-damaging agent. Those that germinated grew slower, and the majority ceased growth within 2 weeks. Pollen from uev1d plants also displayed a moderate but significant decrease in germination in the presence of DNA damage. This report links Ubc13-Uev with functions in DNA damage response in Arabidopsis.
Mesh Terms:
Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, DNA Damage, DNA, Bacterial, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Genetic Complementation Test, Germination, Lysine, Methyl Methanesulfonate, Molecular Sequence Data, Mutagenesis, Insertional, Mutation, Phenotype, Pollen, Polyubiquitin, Protein Binding, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Seeds, Ubiquitin-Conjugating Enzymes, Ubiquitin-Protein Ligases, Ubiquitination
Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, DNA Damage, DNA, Bacterial, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Genetic Complementation Test, Germination, Lysine, Methyl Methanesulfonate, Molecular Sequence Data, Mutagenesis, Insertional, Mutation, Phenotype, Pollen, Polyubiquitin, Protein Binding, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Seeds, Ubiquitin-Conjugating Enzymes, Ubiquitin-Protein Ligases, Ubiquitination
Plant Cell
Date: Jan. 01, 2008
PubMed ID: 18178771
View in: Pubmed Google Scholar
Download Curated Data For This Publication
76608
Switch View:
- Interactions 14