Identification of proteins that interact with NF-YA.
We used the yeast two-hybrid system to show that the serum response factor (SRF) and zinc-fingers and homeobox 1 (ZHXI) proteins interact with the A subunit of nuclear factor-Y (NF-YA). GST pulldown assays revealed that both proteins interact specifically with NF-YA in vitro. Amino acids located between 272 and 564, ... a region that contains two homeodomains, are required for the interaction of ZHX1 with NF-YA. Two different domains of NF-YA, a glutamine-rich region and a serine/threonine-rich region, are necessary for the interactions with ZHX1 and SRF, respectively.
Mesh Terms:
CCAAT-Binding Factor, DNA-Binding Proteins, Genes, Reporter, Homeodomain Proteins, Humans, Nuclear Proteins, Plasmids, Protein Binding, Serum Response Factor, Transcription Factors, Transcriptional Activation, Yeasts, Zinc Fingers
CCAAT-Binding Factor, DNA-Binding Proteins, Genes, Reporter, Homeodomain Proteins, Humans, Nuclear Proteins, Plasmids, Protein Binding, Serum Response Factor, Transcription Factors, Transcriptional Activation, Yeasts, Zinc Fingers
FEBS Lett.
Date: Oct. 22, 1999
PubMed ID: 10571058
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