Dominant negative analogs of NF-YA.

NF-Y is a highly conserved heteromeric CCAAT-binding transcription factor involved in the function of several promoters. The NF-YA subunit contains a domain of high homology to yeast HAP2, which we show to be necessary and sufficient to mediate interactions with the NF-YB subunit and with DNA. Using protein affinity columns ...
derivatized with amino acid substitution mutants, we further dissect this region into two functionally separable subdomains. The subunit association function resides in a 21-amino acid stretch, which is almost perfectly conserved among different species, while interaction with DNA resides in another short segment. We also show that DNA-binding mutants act as dominant repressors of NF-Y-DNA complex formation and of NF-Y-dependent transcription.
Mesh Terms:
Amino Acid Sequence, Base Sequence, CCAAT-Enhancer-Binding Proteins, DNA, DNA-Binding Proteins, Genes, Dominant, Molecular Sequence Data, Mutation, Nuclear Proteins, Oligodeoxyribonucleotides, Sequence Alignment, Structure-Activity Relationship, Transcription Factors
J. Biol. Chem.
Date: Aug. 12, 1994
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