Inhibition of androgen receptor-mediated transcription by amino-terminal enhancer of split.
A yeast two-hybrid assay has identified an androgen-dependent interaction of androgen receptor (AR) with amino-terminal enhancer of split (AES), a member of the highly conserved Groucho/TLE family of corepressors. Full-length AR, as well as the N-terminal fragment of AR, showed direct interactions with AES in in vitro protein-protein interaction assays. ... AES specifically inhibited AR-mediated transcription in a well-defined cell-free transcription system and interacted specifically with the basal transcription factor (TFIIE) in HeLa nuclear extract. These observations implicate AES as a selective repressor of ligand-dependent AR-mediated transcription that acts by directly interacting with AR and by targeting the basal transcription machinery.
Mesh Terms:
Epithelial Cells, Gene Expression Regulation, Hela Cells, Humans, Male, Prostate, Proteins, Receptors, Androgen, Repressor Proteins, Transcription Factors, Transcription Factors, TFII, Transcription, Genetic
Epithelial Cells, Gene Expression Regulation, Hela Cells, Humans, Male, Prostate, Proteins, Receptors, Androgen, Repressor Proteins, Transcription Factors, Transcription Factors, TFII, Transcription, Genetic
Mol. Cell. Biol.
Date: Jul. 01, 2001
PubMed ID: 11416139
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