TOPLESS mediates auxin-dependent transcriptional repression during Arabidopsis embryogenesis.
The transcriptional response to auxin is critical for root and vascular development during Arabidopsis embryogenesis. Auxin induces the degradation of AUXIN/INDOLE-3-ACETIC ACID (AUX/IAA) transcriptional repressors, freeing their binding partners, the AUXIN RESPONSE FACTOR (ARF) proteins, which can activate transcription of auxin response genes. We show that TOPLESS (TPL) can physically ... interact with IAA12/BODENLOS (IAA12/BDL) through an ETHYLENE RESPONSE FACTOR (ERF)-associated amphiphilic repression (EAR) motif. TPL can repress transcription in vivo and is required for IAA12/BDL repressive activity. In addition, tpl-1 can suppress the patterning defects of the bdl-1 mutant. Direct interaction between TPL and ARF5/MONOPTEROS, which is regulated by IAA12/BDL, results in a loss-of-function arf5/mp phenotype. These observations show that TPL is a transcriptional co-repressor and further our understanding of how auxin regulates transcription during plant development.
Mesh Terms:
Amino Acid Motifs, Arabidopsis, Arabidopsis Proteins, DNA-Binding Proteins, Gene Expression Regulation, Plant, Indoleacetic Acids, Models, Genetic, Mutation, Protein Binding, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repressor Proteins, Seedling, Seeds, Transcription Factors, Transcription, Genetic, Two-Hybrid System Techniques
Amino Acid Motifs, Arabidopsis, Arabidopsis Proteins, DNA-Binding Proteins, Gene Expression Regulation, Plant, Indoleacetic Acids, Models, Genetic, Mutation, Protein Binding, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repressor Proteins, Seedling, Seeds, Transcription Factors, Transcription, Genetic, Two-Hybrid System Techniques
Science
Date: Mar. 07, 2008
PubMed ID: 18258861
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