The COOH-terminus of parathyroid hormone-related protein (PTHrP) interacts with beta-arrestin 1B.
Parathyroid hormone-related protein (PTHrP) has a diverse range of proposed biological activities participating in both extracellular and intracellular signaling. In order to identify candidate protein effectors, yeast two-hybrid screens were conducted using mature human PTHrP (residues 1-141) and the COOH-terminus (residues 107-141). Both PTHrP baits interacted with a beta-arrestin 1B ... fragment, an important component of G-protein-coupled receptor desensitization and MAPK signaling. Co-immunoprecipitation, in vitro binding assays and colocalization experiments confirmed this interaction in human cells and this required residues 122-141 of PTHrP. These findings suggest that beta-arrestin 1 acts as an effector for a novel function of PTHrP in cytoplasm.
Mesh Terms:
Arrestins, Base Sequence, Cell Nucleus, Cells, Cultured, Cytoplasm, Humans, Molecular Sequence Data, Parathyroid Hormone-Related Protein, Peptide Fragments, Peptide Hormones, Recombinant Proteins, Two-Hybrid System Techniques
Arrestins, Base Sequence, Cell Nucleus, Cells, Cultured, Cytoplasm, Humans, Molecular Sequence Data, Parathyroid Hormone-Related Protein, Peptide Fragments, Peptide Hormones, Recombinant Proteins, Two-Hybrid System Techniques
FEBS Lett.
Date: Sep. 11, 2002
PubMed ID: 12220636
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