Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.

We have performed a survey of soluble human protein complexes containing components of the transcription and RNA processing machineries using protein affinity purification coupled to mass spectrometry. Thirty-two tagged polypeptides yielded a network of 805 high-confidence interactions. Remarkably, the network is significantly enriched in proteins that regulate the formation of ...
protein complexes, including a number of previously uncharacterized proteins for which we have inferred functions. The RNA polymerase II (RNAP II)-associated proteins (RPAPs) are physically and functionally associated with RNAP II, forming an interface between the enzyme and chaperone/scaffolding proteins. BCDIN3 is the 7SK snRNA methylphosphate capping enzyme (MePCE) present in an snRNP complex containing both RNA processing and transcription factors, including the elongation factor P-TEFb. Our results define a high-density protein interaction network for the mammalian transcription machinery and uncover multiple regulatory factors that target the transcription machinery.
Mesh Terms:
Amino Acid Sequence, Carrier Proteins, Cell Line, Humans, Macromolecular Substances, Molecular Sequence Data, Nucleotidyltransferases, Protein Interaction Mapping, RNA Interference, RNA Polymerase II, RNA Processing, Post-Transcriptional, Ribonucleoproteins, Small Nuclear, Transcription, Genetic
Mol. Cell
Date: Jul. 20, 2007
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